Figure 3 | Laboratory Investigation

Figure 3

From: Expression of CXCL1 in human endothelial cells induces angiogenesis through the CXCR2 receptor and the ERK1/2 and EGF pathways

Figure 3

Chemokine (C–X–C motif) ligand 1 (CXCL1) effects are associated with extracellular-signal-regulated kinase (ERK)1/2 signaling. (a) The phosphorylation status of 18 important signaling proteins was monitored by array hybridization. Modulation of CXCL1 was associated with ERK1/2 in both human umbilical vein endothelial cells (HUVECs) and human dermal microvascular endothelial cells (HDMECs). (b) Western blot analysis shows that the phosphorylation levels of ERK1/2 were reduced when CXCL1 stimulation was absent. Blots were performed three times with similar results. (c) Targeting of ERK1/2 resulted in reduction in cellular viability. PD98059, a mitogen-activated protein kinase kinase (MEK) inhibitor; TWS119, a glycogen synthase kinase 3β (GSK-3β) inhibitor. Data from one representative experiment are presented as mean±s.d., *P<0.05. Ab, antibody; IgG, immunoglobulin G; NS, nonsignificant; rh, recombinant human.

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