Figure 4 | Laboratory Investigation

Figure 4

From: Expression of CXCL1 in human endothelial cells induces angiogenesis through the CXCR2 receptor and the ERK1/2 and EGF pathways

Figure 4

Chemokine (C–X–C motif) ligand 1 (CXCL1) regulates epidermal growth factor (EGF) expression. (a) A polymerase chain reaction (PCR) array was used to assess the expression of 84 genes reportedly related to angiogenesis. Treatment of human umbilical vein endothelial cells (HUVECs) with recombinant human (rh) CXCL1- or CXCL1-neutralizing antibodies altered the expression of several genes, including EGF and CXCL4 (left and right panels). (b) The concentrated conditioned media of HUVECs and human dermal microvascular endothelial cells (HDMECs) subjected to the same treatments was analyzed by western blot. Blots were performed three times with similar results. FGF, fibroblast growth factor; IFN, interferon; IL, interleukin; TGF, tumor growth factor.

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