Figure 2

An immunohistochemical analysis of Notch1 in a rat carcinogenesis model. Representative hematoxylin and eosin (H&E) staining photomicrographs of normal (a), dysplastic (b) and cancerous tissues (c) of the tongue obtained from rats used in a rat carcinogenesis model. Original magnification, × 100. Scale bar=100 μm. (d–f) Representative immunohistochemical stainings of Notch1 at each pathological grade in high-power fields. Tissues obtained from rats used in a rat carcinogenesis model were immunohistochemically stained using an antibody to Notch1. The membranous immunoreactions (brown) were regarded as positive for Notch1. Original magnification, × 400. Scale bar=20 μm. (g–i) Representative immunoreactions of Notch1 and intracellular Notch domain (NICD) in high-power fields. Tissues obtained from rats used in a rat carcinogenesis model were immunohistochemically stained using an antibody to NICD. The nuclear immunoreactions (brown dots) were regarded as positive for NICD. In the cancerous tissues, a majority of oral squamous cell carcinoma (OSCC) cells showed strong immunoreactions for NICD. Original magnification, × 400. Scale bar=20 μm.