Figure 3
From: The pathological significance of Notch1 in oral squamous cell carcinoma
The mRNA and protein expressions of Notch1-related molecules in oral squamous cell carcinoma (OSCC) cell lines. (a) The mRNA (top panel) and protein (bottom panel) expressions of Notch1- and Notch1 signaling-related molecules in OSCC cell lines. OSCC cell lines cultured in the same conditions were harvested and supplied for real-time (RT)-PCR and western blotting. The mRNAs of Notch1- and Notch1 signaling-related molecules were found to be expressed in a majority of OSCC cell lines in RT-PCR. The Notch1 and Notch1 and intracellular Notch domain (NICD) expressions were clearly detectable in 9 of 10 cell lines using western blotting, and the other Notch1-related proteins were also similarly detected. (b) Immunofluorescent staining of Notch1, NICD, Jagged (JAG)1, hairly enhancer of split (Hes)1 and Hes related with YRPW motif (Hey1) in SAS cells. Each protein was detected using specific antibodies. The signals of all molecules (Alexa Fluor® 568, red) were clearly detectable in the membranes or nuclei (4′, 6-diamidino-2-phenylindole (DAPI), blue) of SAS cells. Original magnification, × 400.
