Figure 6

Effects of Notch1 small interfering RNA (siRNA) on SAS cell invasion. (a, b) The effects of tumor necrosis factor (TNF-α) treatment on SAS cell invasion. SAS cells were incubated with either control or Notch1-specific siRNA for 24 h in the presence of TNF-α. The numbers of invaded cells obtained from three independent experiments were counted and statistically analyzed. The photos are representative fields of invasive cells on the membranes. TNF-α markedly activated the invasive properties of the cells. Original magnification, × 100. The bar graphs represent the average numbers of cells on the underside of the membranes. The Y axis shows the numbers of cells invading the underside of the membranes. Mean±s.d. of three independent experiments. **P<0.01, analyzed by Student’s t-test. (c, d) The effects of Notch1 siRNA on TNF-α-dependent SAS cell invasion. The photos are representative fields of invasive cells on the membranes. TNF-α-dependent activation of tumor invasion was significantly inhibited under Notch1 knockdown conditions. Original magnification, × 100. The bar graphs represent the average numbers of cells on the underside of the membranes. The Y axis shows the numbers of cells invading the underside of the membranes. Mean±s.d. of three independent experiments. **P<0.01, analyzed by Student’s t-test.