Figure 10

Cellular distribution of endothelial and fibrotic markers involved in H₂O₂-induced conversion of ECs into myofibroblasts inhibited by transfection of an siRNA against TGF-β1 and TGF-β2. (a–y) Representative images from ECs transfected with an siRNA against TGF-β1 (siTGFβ1) (e–h and q–t), an siRNA against TGF-β2 (siTGFβ2) (i–l and u–y) or a non-targeting siRNA (siCTRL) (a–d and m–p), and incubated in the absence (−) or presence (+) of 10 μM H₂O₂ for 72 h. VE-cadherin or CD31 (red), and FSP-1, α-SMA, or FN (green) were detected. Nuclei were stained using DAPI. Bar scale represents 10 μm (N=3).