Figure 2

Cellular distribution of endothelial and fibrotic markers involved in H₂O₂-induced conversion of ECs into myofibroblasts. (a–p) Representative images from ECs in the absence of H₂O₂ (0 μM H₂O₂) (a, e, i, and m) or in the presence of 0.1 μM H₂O₂ (b, f, j, and n), 1 μM H₂O₂ (c, g, k, and o), and 10 μM H₂O₂ (d, h, l, and p) for 72 h. VE-cadherin or CD31 (red), and FSP-1 or α-SMA (green) were detected. The box depicted in (a-d and i-l) indicates the magnification shown in (e–h and m–p), respectively. Arrows indicate VE-Cadherin (e–h) or CD31 (m–p) labeling at the plasma membrane, whereas arrowheads indicate FSP-1 (g, h) or α-SMA (m–p) staining. Nuclei were stained using DAPI. Bar scale represents 10 μm (N=3).