Table 2 Plasma bile salt species in 0.03% cholic acid-fed mice

From: Hepatic cytochrome P450 deficiency in mouse models for intrahepatic cholestasis predispose to bile salt-induced cholestasis

 

WT (n=3)

Hrn (n=10)

Atp8b1G308V/G308V (n=4)

Atp8b1G308V/G308V/Hrn (n=6)

Abcb4−/− (n=8)

Abcb4−/−/Hrn (n=5)

Plasma total bile salts (μ M)

4.1±2.2

3.9±1.0

187.8±70.1

764.7±264.0

6.3±1.5

840.4±496.0

Species (% of total)

 Tauro-α-muricholate

14.4±8.3

6.3±1.6

10.9±7.8

1.2±0.9

0.7±0.2

2.1±0.6

 Tauro-β-muricholate

11.9±6.9

21.5±5.0

22.8±1.5

12.3±4.8

20.2±2.7

8.7±4.2

 Tauroursodeoxycholate

0.4±0.2

0.3±0.2

1.1±1.0

0.4±0.2

1.7±0.9

4.0±3.9

 Taurocholate

71.3±2.3

52.7±7.3

47.8±16.1

81.9±7.4

46.9 ±7.0

76.4±8.8

 Taurochenodeoxycholate

n.d.

3.1±2.1

0.6±0.5

1.2±1.0

n.d.

1.9±1.2

 Ω-Muricholate

n.d.

0.9±0.7

0.9±0.6

n.d.

0.4±0.4

1.6±1.2

 Taurodeoxycholate

n.d.

13.0±6.1

1.2±1.2

0.3±0.2

13.6±6.9

5.1±4.0

 α-Muricholate

n.d.

0.7±0.7

1.2±0.7

0.1±0.02

n.d.

n.d.

 β-Muricholate

n.d.

n.d.

5.5±3.2

0.2±0.09

n.d.

0.1±0.01

 Cholate

1.9±1.1

1.4±1.4

8.0±4.2

1.6±0.6

n.d.

0.1±0.08

 Chenodeoxycholate

n.d.

n.d.

n.d.

0.7±0.9

16.5±3.9

0.1±0.08

 Deoxycholate

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

Hydrophobicity index

−0.21±0.1

−0.13 ±0.06

−0.20±0.005

−0.06±0.02a

0.03±0.09

−0.06±0.06b

  1. Plasma bile salt species of cholic acid-fed mice were determined by HPLC analyses and are expressed as percentage of total bile salt. For clarity, total plasma bile salt concentrations are indicated. Hydrophobicity indices were calculated using the individual hydrophobicity indices as determined by Heuman.28 Data are given as mean±s.e.m.
  2. aP=0.0016 for Atp8b1G308V/G308V versus Atp8b1G308V/G308V/Hrn.
  3. P=0.49 for Abcb4 −/− versus Abcb4−/−/Hrn using Student’s t-test.; n.d., not detectable.
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