Figure 2
From: A practical guide to induced pluripotent stem cell research using patient samples
A typical work flow of patient iPSC research and tips for individual steps. (1) iPSC generation (∼3 weeks)—multiple clones from multiple patients using non-integrating reprogramming vectors; (2) Quality control (QC) and storage (1–4 weeks)—first by morphology and pluripotency markers, then ideally by gene expression profiling, teratoma formation, karyotyping, exome analysis, and mycoplasma testing; (3) Isogenic controls made by gene editing serve as ideal controls; (4) Differentiation (2–10 weeks)—consult a local iPSC core or colleagues to identify the best available protocols; (5) Disease recapitulation—set realistic goals to demonstrate unique pathological changes in vitro; (6) Study further disease mechanisms—molecular ‘omic’ analyses are often used here. ‘Green’ highlighted parts are usually taken care by a local iPSC core facility (if desired), whereas ‘blue’ highlighted parts will typically be performed by individual investigators.
