Figure 6 | Laboratory Investigation

Figure 6

From: Downregulation of spleen tyrosine kinase in hepatocellular carcinoma by promoter CpG island hypermethylation and its potential role in carcinogenesis

Figure 6

Association of SYK with cytoskeleton and adhesion molecules. Hep3B and Huh7 cells transiently expressing SYK-tGFP were attached on fibronectin-coated coverslip for 1 h, fixed, permeabilized, and stained with Rhodamine Phalloidin (a), anti-tensin2 (b), and anti-vinculin (c). Scale bars represent 10 μm. (d) Lysates (Input), anti-IgG (IP: IgG) or anti-SYK immune complexes (IP: SYK) prepared from Dox-treated Hep3B, Huh7 (induced-SYK) and SNU-739 (endogenous SYK) cells were analyzed by western blotting using anti-SYK, anti-beta-actin, anti-vinculin, and anti-tensin2 antibodies.

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