Figure 5
Knockdown of galectin-1 in pancreatic cancer-associated fibroblasts dramatically reduced cell migration and invasion. Shown are representative immunofluorescent images of CAF11-500 cells transfected with control shRNA (NCC, a) or galectin-1 shRNA (b) stained with anti-galectin1 (green), phalloidin (red), and counterstained with DAPI (blue). (c) Western blot analysis showing decreased expression of galectin-1 following transfection with shRNA constructs. GAPDH is shown as a loading control. (d) Wound healing assay shows reduction of cell movement into the wounded area at 24h post wounding for galectin-1 knockdown cells (bottom right) compared to control (top right). (e) Invasion assay showed a dramatic reduction in the average number of cells per field for galectin-1 knockdown line compared to control (t-test, P-value <0.001).
