Table 3 Reprogramming of mouse somatic cells into miPSCs by transduction with and without transcription factors (TFs) with the aid of small molecules
From: Generation of pluripotent stem cells without the use of genetic material
TFs required a | Somatic cells | PSCs | Small molecules | Efficiency (%) | Reference |
|---|---|---|---|---|---|
Four factors | |||||
OSKM | MEFs | miPSCs | None | 0.02% | |
OSKM | Mouse hepatocyte and gastric epithelial cells | miPSCs | None | 0.01% | |
OSKM | Mouse liver and stomach cells | miPSCs | None | 0.01% | |
OSKM | MEFs | miPSCs | Compound B6, B8, and/or B10 | 0.14–0.35% | |
Three factors | |||||
KSM | Mouse NPCs | miPSCs | None | 0% | |
KSM | Mouse NPCs | miPSCs | BIX | 0.004% | |
OSK | MEFs | miPSCs | None | 0.001–0.0026% | |
OSK | MEFs | miPSCs | VPA | 0.089% | |
OSK | MEFs | miPSCs | CHIR | 0.18% | |
Two factors | |||||
OK | Mouse NPCs | miPSCs | None | 0.004–0.006% | |
OK | Mouse NPCs | miPSCs | BIX | 0.034% | |
OK | Mouse NPCs | miPSCs | PD+CHIR (2i) | 0.0125% | |
OK | MEFs | miPSCs | None | 0.003% | |
OK | MEFs | miPSCs | BIX/BayK | 0.022% | |
OK | MEFs | miPSCs | CHIR | 0.006% | |
OK | MEFs | miPSCs | 616452 | 0.05% | |
OK | MEFs | miPSCs | VPA, CHIR, 616452 | 0.01–0.04% | |
One factor | |||||
O | Mouse NPCs | miPSCs | None | 0.004% | |
O | MEFs | miPSCs | None | 0% | |
O | MEFs | miPSCs | VPA, CHIR, 616452, Parnate | 0.05–0.08% | |
O | MEFs | miPSCs | AMI-5, A-83-01 | 0.02% | |
O | MEFs | miPSCs | Shh, purmorphamine, or oxysterol | 0.11% | |
No factor | |||||
No factor | Mouse skeletal myoblasts | miPSCs | RG108 | 0.11% | |
No factor | MEFs | miPSCs | VPA, CHIR, 616452, Parnate, Forkolin, DZNep | 0.04% | |
No factor | MEFs | miPSCs | VPA, CHIR, 616452, Parnate, Forkolin, DZNep, TTNPB | 0.2% | |
