Figure 3 | Laboratory Investigation

Figure 3

From: Treatment with CA-074Me, a Cathepsin B inhibitor, reduces lung interstitial inflammation and fibrosis in a rat model of polymyositis

Figure 3

HE, Masson’s trichrome staining analyses of lung tissues and western blotting analysis, Immunohistochemical staining analysis of the expression of CB protein levels in lung tissues in a guinea-pig model of PM (10 × 40, n=6–8). Data are means±s.d. F1=sham group, F2=CVB+None group, F3=CVB+Saline group, F4=CVB+CA-074Me group (f: upper panel). Infiltration of many inflammatory cells in lung tissues and fiber breakages were found in the CVB+None group and decreased in the CVB+CA-074Me group (a). The inflammatory score increased in the CVB+None group in comparison with the sham group, and decreased in the CVB+CA-074Me group with significant difference (b). The test of homogeneity of variance showed P>0.1, ANOVA test was applied. The histological lesion (the fibrosis score) increased significantly in the CVB+None group in comparison with the sham group, and decreased in the CVB+CA-074Me group (c). The test of homogeneity of variance showed P<0.1, nonparametric test was applied. The collagen area in lung tissues increased in the CVB+None group in comparison with the sham group, and decreased in the CVB+CA-074Me group with significant difference (d). The test of homogeneity of variance showed P>0.1, ANOVA test was applied. The expression of CB protein levels was done by western blotting analysis in different groups, the prominent band at the molecular size of ∼27 kDa represented the single-chain form of CB (e: upper panel); The gray scale value of CB in different groups was analyzed (e: lower panel). The test of homogeneity of variance showed P<0.1, nonparametric test was applied. Lung tissues containing yellow granulation (arrow showing) in the endochylema or nucleus were considered as positive. The test of homogeneity of variance showed P<0.1, nonparametric test was applied. The number of CB-positive cells increased in the CVB+None group in comparison with the sham group, and decreased in the CVB+CA-074Me group with significant difference (f: lower panel). *P<0.05 in comparison with the sham group, #P<0.05 in comparison with CVB+CA-074Me group.

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