Figure 5 | Laboratory Investigation

Figure 5

From: Glomerular parietal epithelial cell activation induces collagen secretion and thickening of Bowman’s capsule in diabetes

Figure 5

PECs produce and secrete collagen upon stimulation with AGEs, TGF-β1, and high glucose. Stimulation for only 24 h revealed no changes in collagen secretion into the supernatant (a), whereas significantly less collagen was detected in the cells upon stimulation with TGF-β1 (5 ng/ml) for 24 h (c) as assessed by the collagen assay (Sirius Red collagen assay kit, Biocolor). Following the stimulation for 48 h, the PECs produce and secrete significant higher amounts of collagen (b and d). (e) PECs were stimulated with AGEs (100 μg/ml), TGF-β1 (5 ng/ml), and high glucose (30 mM) for 24, 48, and 72 h. Detection of collagen IV using western blot revealed an upregulation of collagen type IV at all time points following stimulation with either AGEs, TGF-β 1, or high glucose relative to the β-actin expression. Displayed are means with s.e.m. *P<0.05 versus control; **P<0.01 versus control; ***P<0.001 versus control. AGEs, advanced glycation end products; PECs, parietal epithelial cells; TGF-β, transforming growth factor-β.

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