Figure 4 | Laboratory Investigation

Figure 4

From: Upregulation of integrin β4 promotes epithelial–mesenchymal transition and is a novel prognostic marker in pancreatic ductal adenocarcinoma

Figure 4

Overexpression of integrin β4 (ITGB4) promotes cell motility and epithelial–mesenchymal transition (EMT) in pancreatic cancer cells. (a) Western blot analyses using lysates of CFPAC-1 and AsPC-1 cells treated with small interfering (si)ITGB4a, siITGB4b and negative control small interfering RNA (siRNA). (b) Migration and invasion assays showed significantly reduced cell migration and invasion in CFPAC-1 and AsPC-1 cells by knockdown of ITGB4. (c) Western blot analyses using lysates of CFPAC-1 clones stably overexpressing ITGB4 (CF/FL-1 and CF/FL-2), control cells transfected with empty vectors (vector-1 and vector-2), and parental CFPAC-1 cells. (d) Cell scattering was noted in CFPAC-1 cells with ITGB4 overexpression compared with control cells. (e) Numbers of migrated and invaded cells increased stepwise with the expression level of ITGB4. Asterisks indicate significance by the t-test (P<0.05). (f) Semiquantitative reverse transcription-PCR (RT-PCR) analyses for EMT-related transcriptional factors in CFPAC-1 transfectants.

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