Figure 1
From: Endothelial cells enhance the in vivo bone-forming ability of osteogenic cell sheets
Subcutaneous transplantation of rat bone marrow stromal cell (rBMSCs) osteogenic cell sheets cocultured with human umbilical vein endothelial cells (HUVECs). rBMSCs were isolated from rat’s femurs, expanded, and seeded in thermoresponsive dishes. After 17 days of culture in osteogenic medium, HUVECs were seeded on top of the confluent rBMSCs. Both cells were cocultured for further 4 days. CSs were retrieved from the dishes by low temperature treatment. Monocultured CSs were stacked on top of cocultured CSs and implanted on the dorsal flap of nude mice. A silicon membrane was used to cover the CSs and the flap was closed and sutured.
