Figure 2
From: Endothelial cells enhance the in vivo bone-forming ability of osteogenic cell sheets
Alizarin red staining (a) and human CD31 immunostaining (c, d) of osteogenic cell sheets cocultured with human umbilical vein endothelial cells (HUVECs) just before retrieval from thermoresponsive dishes. Rat bone marrow stromal cells (rBMSCs) after 21 days of osteogenic differentiation (a, left side dish) and cultured in basal medium (a, right side dish). The nuclei of all cells are stained blue with 4',6-diamidino-2-phenylindole (DAPI) nuclear staining (b) and HUVECs (human CD31-positive) distribution is depicted in green. A merge micrograph of (b) and (c) is presented in (d).
