Figure 5
From: Endothelial cells enhance the in vivo bone-forming ability of osteogenic cell sheets
Immunohistological characterization of retrieved implants after implantation of the cocultured CS (a, c) or monoculture CS (b, d) constructs. Retrieved constructs were immunostained for SRY (a, b) and osterix (c, d). Quantitative RT-PCR analysis (e) and calcium quantification (f) were performed in retrieved constructs, 1 week after implantation. In (e), osteocalcin (OC), osterix (Osx) and collagen I (Col I) transcripts were quantified in both the cocultured CS and control cell sheet constructs (*P<0.05 relating to control values). Calcium was quantified using the o-cresolphtalein-complexon method and values were normalized with tissue mass (*P<0.05 relating to coculture cell sheets).
