Figure 6
Src-deficient mice and PP2 attenuated lung stretch-induced bronchial epithelial apoptosis. Representative micrographs with transmission electron microscopic image (a, × 6000) (n=3 per group) and TUNEL staining (b, c, × 400) (n=5 per group) of the paraffin lung sections 5 days after bleomycin treatment were from the nonventilated control mice and those subjected to VT at 6 ml/kg or at 30 ml/kg for 5 h with room air. Apoptosis-positive cells were quantified as the average number of epithelial cells with bright green signals per bronchiole, which were counted from 10 nonoverlapping fields of each section (n=5 per group). PP2 2 mg/kg was given intraperitoneally 30 min before ventilation. Apoptotic cells are identified by asterisks or arrows. A bright green signal indicates positive staining of apoptotic cells, and shades of dull green signify non-reactive cells. Scale bars represent 500 nm or 20 μm. *P<0.05 vs the nonventilated control mice with bleomycin pretreatment; †P<0.05 vs all other groups. TUNEL=terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling.
