Table 1 Experimental design and numbers of animals per group
EBD, lung water (5 h) | PAI-1, TGF-β1, collagen, total protein (5 h) | Src protein (1 h) | μCT (5 h) | EM (5 h) | Isolated fibroblasts (5 h) | IF, IHC, Trichrome stain, TUNEL (5 h) | |
|---|---|---|---|---|---|---|---|
Control (wild type without bleomycin) | 5 | 5 | 5 | 5 | 3 | 5 | 5 |
Control (wild type with bleomycin)− | 5 | 5 | 5 | 5 | 3 | 5 | 5 |
VT 6 ml/kg (wild type with bleomycin) | 5 | 5 | 5 | 0 | 0 | 0 | 5 |
VT 30 ml/kg (wild type with bleomycin) | 5 | 5 | 5 | 5 | 3 | 5 | 5 |
VT 30 ml/kg (wild type with bleomycin)+PP2 | 5 | 5 | 5 | 5 | 3 | 5 | 5 |
VT 30 ml/kg (Src+/− with bleomycin) | 5 | 5 | 5 | 5 | 3 | 5 | 5 |
