Figure 3 | Laboratory Investigation

Figure 3

From: Micheliolide, a new sesquiterpene lactone that inhibits intestinal inflammation and colitis-associated cancer

Figure 3

PTL and MCL inhibit LPS-induced cytokine secretion by macrophages in a concentration-dependent manner. (a–c) RAW 264.7 cells were treated with 0, 1, 2, 5, or 10 μM PTL or MCL for 8 h and then stimulated with LPS (10 ng/ml). Total RNA was extracted and purified, and the expression of IL-1β (a), IL-6 (b), and TNFα (c) were quantified by qRT-PCR. (d) RAW 264.7 cells were stimulated with LPS (10 ng/ml) for 1 h and then treated with 0, 1, 2, 5, or 10 μM PTL or MCL for 8 h. TNFα secreted into the supernatant was assayed by ELISA. *P<0.05 compared with cells stimulated with LPS-only.

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