Figure 5

Resident liver immune cells and ConA-induced cytokine profiles are similar between JAX livers and TAC livers. (a) Liver MNC were isolated from naïve JAX BALB/c mice and naïve TAC BALB/c mice and total CD45+ cells quantified by FACS. Each symbol represents a single mouse, and results are aggregated in box-and-whiskers plots. (b, c) Frequencies of liver (b) lymphoid (T (TCRβ+), CD4+ T, CD8+ T, NKT (CD1d+TCRβ+), NK (CD49b+TCRβ−), B (CD19+)) and (c) myeloid (monocytic/macrophage (CD11b+Gr1+ and CD11b+Gr1−), neutrophils (CD11b-Gr1+), dendritic cells (CD11c+)) cells in naïve JAX mice and TAC mice were obtained. Data are representative of three independent experiments. *P<0.05, **P<0.01, ***P<0.001. (d) Cytokine profiles of liver lysates from BALB/c mice collected before (0 h) and after (0.75 h, 1.5 h, 3 h, 6 h, 24 h) ConA injection. Cytokines were quantified by cytokine bead array. N=6 mice per vendor and time point. P-values (ANOVA) are shown for each cytokine. y axes are log₂ scale.