Figure 7

Microbiota regulates sensitivity to Fas-induced liver injury through an MyD88-dependent pathway. (a) Fecal matter was collected from BALB/cJ mice before and after 2 weeks of antibiotics treatment. DNA was extracted and 16S qRT-PCR was used to assess the fold bacterial load reduction. Each point represents a single mouse. (b) Representative images show that treatment with antibiotics (top two ceca) leads to cecal enlargement compared with control treatment (bottom two ceca). (c) After 2 weeks of antibiotic (ABX) treatment and recovery for 2 days, treated (n=19) and control (n=12) JAX BALB/c mice were injected with Jo-2 (0.1 μg/g). ALT was measured at 6 h (left) and 24 h (right) post injection. (d) Five-week-old germ-free (GF) Swiss-Webster (TAC:SW) mice were orally gavaged with fecal contents of age-matched JAX mice. Five weeks later, reconstituted mice (GF-R) and age-matched GF mice were challenged with Jo-2, and killed at 24 h. Plasma ALT levels and histopathological scores are shown. (e) Six- to eight-week-old homozygous Myd88-deficient (−/−), heterozygous (+/−), and homozygous wild-type (+/+) mice that were born and raised as littermates were injected with Jo-2. ALT and representative H&E (scale bar=100 μm) are shown at 6 h post injection. ALT data are aggregated from three litters. *P<0.05; ***P<0.001.