Figure 4 | Laboratory Investigation

Figure 4

From: Inhibition of notch signaling pathway prevents cholestatic liver fibrosis by decreasing the differentiation of hepatic progenitor cells into cholangiocytes

Figure 4

Inhibition of Notch signaling reduced HPC differentiation to bile duct epithelial cells in vivo. (a) Immunostaining of CK19 (× 200). (b) Immunostaining of OV6 (× 200). (c) Double immunofluorescent staining of CK19 (red) and Sox9 (green) merged (× 200). (d) CK19, OV6, Sox9, and EpCAM mRNA were measured with RT-PCR and normalized to GAPDH mRNA (n=6 per group). (e) CK19, OV6, Sox9, and EpCAM protein were quantified with immunoblotting, and (f) densitometric analysis of protein bands normalized to GAPDH (n=6 per group). *P<0.05, **P<0.01. BDL, single bile duct ligation group; DAPT, BDL plus DAPT group; sham, sham group.

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