Figure 5 | Laboratory Investigation

Figure 5

From: Inhibition of notch signaling pathway prevents cholestatic liver fibrosis by decreasing the differentiation of hepatic progenitor cells into cholangiocytes

Figure 5

Inhibition of Notch signaling reduced HPC differentiation to bile duct epithelial cells in vitro. (a) Immunofluorescent staining of CK19 (red) and DAPI (blue) (× 400). (b) CK19, Sox9, and EpCAM mRNA were measured with RT-PCR and normalized to GAPDH mRNA (n=6 per group). (c) CK19, Sox9, and EpCAM protein was quantified by immunoblotting and normalized to GAPDH, and (d) densitometric analysis of protein bands (n=6 per group). (e) Notch1, Notch2, Notch3, JAG1, JAG2, DLL3 and HES1 mRNA were measured with RT-PCR and normalized to GAPDH mRNA (n=6 per group). *P<0.05, **P<0.01. C, control group; DAPT, SB plus DAPT (25 μM) group; SB, sodium butyrate group (3.75 mM).

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