Figure 6 | Laboratory Investigation

Figure 6

From: Inhibition of notch signaling pathway prevents cholestatic liver fibrosis by decreasing the differentiation of hepatic progenitor cells into cholangiocytes

Figure 6

Inhibition of the Notch signaling pathway reduced the progression of liver fibrosis. (a) Notch-1, Notch-2, Notch3, Notch4, JAG1, JAG2, DLL1, RBPJк, and Numb, protein bands on the left (immunoblot), and the histogram depicts the densitometric analysis of protein bands (n=6 per group). (b) Notch-1, Notch-2, Notch3, Notch4, JAG1, JAG2, DLL1, RBPJк, and Numb mRNA were measured via RT-PCR and normalized to GAPDH mRNA (n=6 per group). (c) Sirius Red staining (× 100). (d) α-SMA immunostaining (× 200). (e) Hydroxyproline content. (f) α-SMA, TNF-α, TGF-β1, MCP-1, Col(1), and Col(4) mRNA were measured by RT-PCR and normalized to GAPDH mRNA (n=6 per group). *P<0.05, **P<0.01. BDL, single bile duct ligation group; DAPT, BDL plus DAPT group; sham, sham group.

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