Figure 4 | Laboratory Investigation

Figure 4

From: Vascular endothelial growth factor receptor-3 is a novel target to improve net ultrafiltration in methylglyoxal-induced peritoneal injury

Figure 4Figure 4

In immunohistochemical analyses of the diaphragm, adenovirus-expressing sVEGFR-3 suppressed LYVE-1–positive lymphatic vessels but not expression of CD31-positive vessels, type III collagen or CD68-positive cells. The diaphragm of mice treated with Adeno-Lac Z (Lac Z) or with Adeno-sVEGFR-3-Ig (R3-Ig) was immunohistochemically analyzed for: (a) LYVE-1–positive lymphatic vessels, (b) CD31-positive vessels, (c) deposition of type III collagen, and (d) CD68-positive macrophages. (each group, n=6). Scale bars, 100 μm. Lac Z: treatment with Adeno-LacZ, R3-Ig: treatment with Adeno-sVEGFR-3-Ig. LYVE-1, lymphatic vessel endothelial hyaluronan receptor-1; ND, not detected; NS, not significant; PD, peritoneal dialysis; RT–PCR, reverse transcription–PCR; sVEGFR-3, soluble vascular endothelial growth factor receptor-3.

Back to article page