Figure 4
From: Inhibition of dipeptidyl peptidase IV protects tacrolimus-induced kidney injury
Effect of MK0626 (M) on oxidative stress during tacrolimus (TAC)-induced renal injury. (a) Immunohistochemistry for 8-hydroxy-2′-deoxyguanosine (8-OHdG) in tissue sections. Intense nuclear expression of 8-OHdG is shown in the TAC group, and the administration of MK0626 reduces its expression levels. (b) Urinary 8-OHdG excretion per day. The TAC-induced 8-OHdG excretion is lowered by the coadministration of MK0626. (c and d) Immunoblot analysis of manganese superoxide dismutase (MnSOD), heme oxygenase-1 (HO-1, inducible form), and HO-2 (constitutive form) in the renal cortex from the experimental animals. The relative optical densities of bands in each lane were normalized to each β-actin band from the same gel. Note that combined treatment with MK0626 restored their expression compared with TAC. Magnification, × 400. n=8 per group. #P<0.05 vs VH; $P<0.05 vs TAC.
