Figure 5
Genetic deletion of LRP1 in HSCs reveals its contribution to resolution of hepatic injury and matrix deposition. LRPflox/flox (WT) and LRPflox/flox;SM22-Cre+ (LRP1 cKO) mice treated with CCL4 as described in MATERIALS AND METHODS. Liver tissue and serum were collected at days 4 and 6 after the last dose. (n=3 animals for each time point and genotype). (a) Representative confocal images of double immunolabeling with anti-LRP1 and anti-α-SMA antibodies of sections from day 4 post-injury. Area inside the dotted squares is shown in the insert. Yellow staining indicates co-localization of LRP1 and α-SMA. Scale bars, 50 μm. (b) Serum ALT values in WT and LRP1 cKO mice at 4 and 6 days after the last CCL4 injection. (c and d) Representative images (left) and quantification (right) for immunohistochemistry for α-SMA (c) and Collagen I (d) at the indicated time points after injury. Sections from WT and cKO mice on matching days were stained, imaged, and analyzed together. Error bars±s.e.m. *P<0.05, **P<0.01, ***P<0.001, unpaired Student’s t-test. Scale bars, 50 μm.
