Figure 6
Ischemic legs have increased high-mobility group box 1 (HMGB-1) expression. HMGB1 was quantified both using immunohistochemistry and immunoblotting of the ischemic and non-ischemic legs. (a) Representative images of slides stained for HMGB-1 (red) so increased HMGB1 in the ischemic leg. (red=HMGB1 and blue=DAPI) (b) Expression of HMGB1 in the muscle was quantified by western blot. (n=8, *P<0.0001) (c) Representative images show that HMGB-1 expression is not different between groups. (d) Western blotting confirms no difference in HMGB1 expression between groups (n=4) (e) Co-staining reveals that HMGB1 is expressed in smooth muscle cells, endothelial cells, and macrophages (green=HMGB1, blue=DAPI, and red=α-smooth muscle actin, lectin, or MAC387). IL, ischemic leg; KO, knockout; NIL, non-ischemic leg; RAGE, receptor for advanced glycation end products; WT, wild type; WT+DM, wild-type mice with diabetes.
