Figure 6 | Laboratory Investigation

Figure 6

From: Maresin1 stimulates alveolar fluid clearance through the alveolar epithelial sodium channel Na,K-ATPase via the ALX/PI3K/Nedd4-2 pathway

Figure 6

MaR1 enhanced Na,K-ATPase expression by western blotting and Na,K-ATPase activity in LPS-induced ALI in vivo. MaR1 (200 ng/kg) was administered to Sprague–Dawley rats 8 h after LPS (14 mg/kg) stimulation through caudal vein or via alveolar instillation, ventilating for 60 min, and the right lung tissue was collected to measure the protein expression of Na,K-ATPase-α1 and β1 subunits by western blotting (a). Western blot data were replicated >3 times and the Na,K-ATPase activity in lung tissue homogenate was detected by kits (b). The control group, equivalent volume of saline. Data are presented as mean±s.e.m. n=8. *P<0.05, **P<0.01 versuscontrol group; #P<0.05, ##P<0.01 versusLPS group.

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