Figure 3
From: Larger core size has superior technical and analytical accuracy in bladder tissue microarray
Image processing. A digital RGB JPEG image of individual cores or a whole-slide section was processed and analyzed in ImageJ. (a) The RGB image is split into the individual R, G, and B channels. The individual gray-scale images are processed as follows: (i) ‘Thresholding’ to display the desired pixel intensity, (ii) ‘Fill Holes’ to fill empty spaces within non-uniform nuclei, (iii) ‘Watershed’ segmentation to separate partially overlapping nuclei. The ‘Analyze Particle’ function is subsequently applied to quantify the number of nuclei identified in each of the Red channel (Ki67+ nuclei) and Blue channel (All nuclei). (b) The ImageJ workflow.
