Figure 1 | Laboratory Investigation

Figure 1

From: Reversal of maladaptive fibrosis and compromised ventricular function in the pressure overloaded heart by a caveolin-1 surrogate peptide

Figure 1

CSD reverses the effect of TAC on HSP47 and Collagen I levels. Mice were treated as described in the Methods. For Sham control, samples from 6 weeks after surgery were used. LV tissue was used for histochemical analyses or extracted with RIPA buffer and the resulting soluble and insoluble fractions used in western blotting experiments. (a) The RIPA soluble protein fractions from mice receiving the indicated treatments were analyzed by Western blotting using antibodies against HSP47 and GAPDH (loading control). (b) LV from the indicated mice (6 weeks after surgery) were stained with anti-HSP47 (red), and co-stained with DAPI (blue) to detect nuclei. The graph quantifies HSP47 staining intensity per field in arbitrary units. Graph data were obtained from 10 fields per slide, three to four mice per category. (c) The RIPA insoluble protein fraction from mice receiving the indicated treatments were Western blotted using antibodies against Collagen I and actin (loading control). (d) LV tissue from the indicated mice harvested 6 weeks after surgery was stained with Picrosirius red. The graph quantifies collagen volume fraction, calculated from photomicrographs using Sigma Scan Pro-5. At least 25 fields per mouse, two mice per category were used. **P<0.01, TAC vs Control; ^P<0.05, TAC+CSD vs TAC+Vehicle; ^^P<0.01, TAC+CSD vs TAC+Vehicle.

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