Figure 6

CSD inhibits eNOS activation. Mice were treated as described in the Methods. For Sham control, samples from 6 weeks after surgery were used. LV tissue was extracted with RIPA buffer and the resulting soluble fraction used in Western blotting experiments using antibodies against phospho-eNOS-S1177 and total eNOS. Thin arrow indicates the position of dimeric eNOS (280 kDa), open arrow indicates monomeric eNOS (140 kDa). Similar results were obtained in independent experiments.