Figure 1

Scar VFFs exhibit increased proliferation at early passages. (a) Cell count data showing greater proliferation of scar VFFs compared with naïve cells after 6 days (d) incubation at P1–P3. Corresponding population doubling times (in hours (h)) are also shown. (b) Representative ICC data showing Ki-67 (red) and CC3 (green) expression in naïve and scar VFFs at P1. Nuclei are counterstained with DAPI (blue). Ki-67⁺ cells were more abundant in the scar, compared with naïve, condition; no difference was observed for CC3. White arrows indicate Ki-67⁺ or CC3⁺ fibroblast nuclei. All experiments were performed with n=4 biological replicates per condition. Data are presented as mean±s.e.m. *P<0.05 in a. Scale bar=100 μm in b.