Figure 4 | Laboratory Investigation

Figure 4

From: Hypoxia-stimulated cardiac fibroblast production of IL-6 promotes myocardial fibrosis via the TGF-β1 signaling pathway

Figure 4Figure 4

Overexpression of IL-6 induced FBs differentiation into myofibroblasts and fibrosis under hypoxia. (a) Quantitative analysis of α-SMA and collagen I mRNA expression in FBs treated with IL-6 in the presence or absence of the inhibitors of IL-6, TGF-β1 and MMP2/MMP9. P<0.05: *vs the CON fibroblast, †vs IL-6 overexpressing fibroblast (n=10, each group). (b) Representative images of immunofluorescence staining for α-SMA (red) and collagen I (green) in FBs treated as mentioned previously. Nuclei were stained with DAPI (blue). Scale bar: 50 mm.

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