Figure 3 | Laboratory Investigation

Figure 3

From: Relationship between tumor-associated macrophage subsets and CD47 expression in squamous cell carcinoma of the head and neck in the tumor microenvironment

Figure 3

(a) CD47 expression on HSC-3 was more effectively, but not completely, knocked down by CD47 siRNA than by negative siRNA. Gray filled line: stained with isotype control Ab; Green: CD47 staining of HSC-3 without any siRNA; Blue: CD47 expression with negative siRNA; Red: CD47 expression with CD47 siRNA. (b) Slight increases were observed in the phagocytosis of HSC-3 by the induced M1 subset (black bar, P=0.08), but not by the induced M2 subset (white bar), with the knockdown of CD47 using siRNA in vitro. Summary experiments were repeated in triplicate. Whole actual cell counts for analyses were 8404±1715 of M1 and 9683±839 of M2.

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