Figure 4 | Laboratory Investigation

Figure 4

From: Relationship between tumor-associated macrophage subsets and CD47 expression in squamous cell carcinoma of the head and neck in the tumor microenvironment

Figure 4

Demonstrative IHC staining of CD47 (a–c), CD68 (d), and CD163 (e) in OSCC tissue magnified by × 400. CD47 staining was evaluated when the percentages of stained tumor cells in an entire lesion were <25% as (−). (a), 25–75% as (±) (b), and >75% as (+) (c). (a) No stained cancer cells, but positive red blood cells. (b) The upper left tumor population was CD47−, whereas the upper right population was CD47+ in a membranous manner. (c) Most cancer cells were CD47+. More than 4 areas of a representative field adjacent to cancer cells were counted at × 400 magnification for CD68+ (d) and CD163+ (e) macrophages and the average was calculated. Each staining of CD44, CD68, and CD163 was from sequential sections.

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