Figure 1

The protective effect of IPo on IIR-induced Lung injury. (a) Histopathologic changes of mice lung under a light microscope (× 400, H&E staining). Forty-five minutes of superior mesenteric artery occlusion followed by 120 min reperfusion caused an excessive alveolar interstitial edema, telangiectasis, hemorrhage, thickening of the alveolar wall, and infiltration of inflammatory cells into the interstitial and alveolar spaces (IIR). These features were dramatically ameliorated after treated with IPo. There were minimal edema, hemorrhage, and inflammatory after IPo. Scale bar, 20 μm. (b) The pulmonary electron microscopic evaluation. Forty-five minutes of superior mesenteric artery occlusion followed by 120 min reperfusion caused a swollen of the basement membranes of alveolar epithelium and capillary endothelium, and reduced or even vanished the electronic density. With the swollen of the mitochondria, the empty of osmiophilic lamellar bodies, and the lodged and disappeared cellar ridge caused by IIR were observed in type II alveolar cells. Moreover, the conjunctions between the alveolar epithelial cells and the capillary endothelial cells were injured with gaps (IIR), which are significantly different from control (group S, S). These features dramatically diminished after treating with IPo (IPO). Scale bar, 2000 nm. (c) Summary of ALI scores in different groups. ALI scores significantly increased after IIR injury. However, IPo treatment can significantly decrease the ALI induced by IIR injury. Arrows indicate. (d, e) The effects of IPo on the pulmonary microvascular permeability (e) and lung tissue wet/dry weight ratios (f) in lung tissue of mouse. Data presented as mean±s.d., n=10. *P<0.05 vs S group, ^#P<0.05 vs IIR group, one-way ANOVA and Tukey’s post hoc test.