Table 1 Characteristics of immortalized SC lines
From: Immortalization of human normal and NF1 neurofibroma Schwann cells
Immortalized SC line (transduced with lentiviral-hTERT and -mCdk4, unless otherwise specified) | Sex | Germline NF1 mutation2 | Somatic NF1 mutation2 | Passage number (p) at which primary culture senesces | Passage number (p) at transduction | Do cells require laminin, neuregulin (NRG)? | Number of passages (p) in culture | Immortal S100B category (1≈all pos; 4=all neg.) | Primary culture S100B category (1≈all pos; 4=all neg.) | Cell morphology (may vary with confluency) (normal SC are spindle shaped) | Proliferation % BrdU 3/4th confluent: primary, immort (passage) | Doubling time in hours (passage) | Do cells form soft agar colonies? (yes/no, passage) | Cells display contact inhibition? (primary, immortalized) | Cells form tumor in sciatic xenograft? | % TUNEL positive (passage) |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
ipNF95.11b C/T plexiform (transgenes from retrovirus) | M | c.1756delACTA | LOH1 | p11 | p8 lenti-TERT, p11 retro-mCdk4 | No, no | >p50 | 1 | 1 | Spindle | 32, 21 (p40) | 34.1 (p33) | No p30 | Yes, no | n.d. | 3.4 (p43) |
ipNF95.11b C same plexiform (retrovirus-mCdk4) | M | c.1756delACTA | LOH1 | p11 | p8 retro-TERT, p20 retro-mCdk4 | No, no | >p50 | 1 | 1 | Fibroblastic and spindle | 32, 18 (p53) | 30.7 (p44) | No p38 | Yes, yes | n.d. | 9.7 (p54) |
ipNF95.6 plexiform | M | R816X | R2237X | p21 | p9 | No, no | >p50 | 4 | 3 | Fibroblastic and spindle | 63, 47 (p47) | 45.0 (p48) | No p38 | Yes, yes | Yes, small | <1 (p51) |
ipNF05.5 plexiform | M | c.3456_3457 insA | LOH 1 | p7 | p4 | No, no (prefers laminin; grows faster with NRG) | >p50 | 1 | 1 | Fibroblastic and spindle | 69, 86 (p18) | 37.7 (p15) | No p22 | Not done | n.d. | 0.3 (p48) |
ipNF05.5 (six-clone mix from ipNF05.5) | M | c.3456_3457 insA | LOH 1 | p7 | p4, clones isolated at p11 | No, no (prefers laminin; grows faster with NRG) | Slows ~p31, +NRG = >p47 | 1 | 1 | Spindle | 69, 82 | 40.3 (p19) | No p25 | Yes, yes | n.d. | 6.3 (p26) |
ipNF06.2A plexiform | F | G848W | Unknown | p15 | p5 | No, no | >p50 | 4 | 3 | Spindle | 49, 32 (p21) | 26.5 (p20) | No p22 | Yes, no | No | 5.4 (p45) |
ipnNF95.11C heterozygous | M | c.1756delACTA | n/a | p11 | p6 | No, no | >p50 | 4 | 1 | Epithelial/ variable | n.d., 50 (p20) | 38.8 (p19) | No p20 | Partial, no | n.d. | 8.0 (p30) |
ipn02.3 2λ normal | F | n/a | n/a | p10 | p5 | No, no | >p50 | 3 | 1 | Spindle | 20, 40 (p79) | 33.4 (p77) | No p75 | Yes, yes | No | 5.8 (p53) |
ipn02.8 normal | F | n/a | n/a | p10 | p3 | No, no | >p50 | 3 | 1 | Fibroblastic | 61, 15 (p21) | 25.6 (p21) | Yes p20 | No, no | n.d. | 45.8 (p19) |
ipn97.4 normal | M | n/a | n/a | p12 | p6 | No, no | >p50 | 1 | 1 | Epithelial | 49, 33 (p20) | 24.7 (p18) | Yes p17 | Yes, no | n.d. | 44.0 (p20) |
sipnNF95.12B heterozygous | F | L216P | n/a | p10 | p1 | Yes, yes | p21 | 4 | 1 | Spindle | n.d. | 82.4 (p18) | No p17 | Yes, partial | n.d. | 11.3 (p18) |
ipnNF09.4 heterozygous | M | c.3456_3457 insA | n/a | p5 | p3 | Yes, yes | >p47 | 1 | 1 | Fibroblastic and spindle | 50, 19.7 (p25) | 59.9 (p18) | No p17 | Yes, yes | n.d. | 9.0 (p46) |
ipNF00.6 plexiform | F | gene deletion (>1 Mb) | Unknown | p10 | p5 | Yes, yes | >p50 | 2 | 1 | Spindle | 65, 27 (p21) | 51.4 (p21) | No p19 | Yes, yes | No | n.d. |
ipNF03.3 plexiform | M | c.4269 G>A in-frame exon skip | Unknown | p7 | p4 | No, no | >p50 | 1 | 2 | Fibroblastic | 20, 67 (p20) | 39.2 (p18) | Yes p17 | Yes, no | Yes | 44 (p48) |
ipNF04.4 plexiform | F | R2237X | LOH 1 | p8 | p5 | No, no | >p50 | 1 | 2 | Epithelial | 22, 41 (p18) | 26.6 (p25) | Yes p24 | Yes, no | Yes | 40 (p20) |
