Figure 5
From: Isolation and characterization of hepatic mast cells from cholestatic rats
Characterization of isolated hepatic mast cells by (a) immunofluorescence and (b) real-time PCR for mast cell markers. (a) Double immunofluorescence was performed in cell smears from isolated mast cell preparations for the following markers: FCɛR1, chymase, and tryptase along with DAPI. Isolated mast cell preparations express FCɛR1, chymase, and tryptase. (b) By real-time PCR the expression of c-kit, FCɛR1, chymase, tryptase, RMCP-I, and RMCP-II are upregulated in isolated mast cells compared with total liver RNA. Images were obtained on a Leica Confocal Microscope and are × 20 magnification. Data are represented as mean±s.e. of six experiments. *P<0.05 versus total liver.
