Figure 1

Schematic diagram of the experimental procedure to test the effects of bleaching techniques using different buffers (Tris-HCl (pH 10), PBS (pH 7.4), or Tris/Tricine/SDS (pH 8.2)), dilutions of H₂O₂ (0.1%, 0.5%, or 1.0%), and incubation periods (2, 5, 10, 15, or 30 min) at 80 °C.