Figure 2 | Laboratory Investigation

Figure 2

From: A melanin-bleaching methodology for molecular and histopathological analysis of formalin-fixed paraffin-embedded tissue

Figure 2

Melanin pigment concentration over time for different dilutions of H2O2 (0.1, 0.5, and 1.0%) with different buffered bleaching solutions (Tris-HCl, PBS, or Tris/Tricine/SDS) at 80 °C. (a) Effect of melanin bleaching using 0.1, 0.5, or 1.0% H2O2 in Tris-HCI (pH 10), PBS, or Tris/Tricine/SDS buffer. Melanin pigments were measured using image analysis software (Visiopharm). Black triangles indicate melanin pigment. (b) H&E images of 0.5% H2O2 diluted with Tris-HCI, PBS, or Tris/Tricine/SDS buffer (scale bar: 100 μm).

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