Figure 6

RNA quantity and quality with different melanin-bleaching methods. (a) Quantity of RNA extracted from melanin-bleached tissue specimens was measured using the RiboGreen kit. RNA-extraction yield is expressed as the mean of three replicates (mean±s.d.). Black bars and clear bars represent the result of no bleaching (▪) and bleaching (□) methods, respectively. (b) To compare the quality of RNA extracted under each condition we used an electropherogram to overlay four different conditions, including non-bleaching. (c) RNA integrity value was represented by the PERM. Black bars and clear bars represent the result of non-bleaching (▪) and bleaching (□) methods, respectively. (d) Representative electropherogram of multiplex PCR amplification. The human housekeeping genes set-2 was used for multiplex PCR reactions. Insets show high magnification of the areas indicated with boxes. NB, non-bleaching.