Figure 5

Inhibitory effect of tenascin-C siRNA on HMVEC proliferation and tube formation, induced by conditioned medium of hRPE cells treated with TGF-β2. (a) Tenascin-C expression in the conditioned medium of hRPE cells transfected with tenascin-C siRNA. Following transfection with tenascin-C or control siRNA (10 nM), hRPE cells were treated with or without TGF-β2 (10 ng/ml) for 24 h (mean±s.e.m.; n=4 per group). Tenascin-C siRNA significantly reduced the concentration of tenascin-C in the conditioned medium of hRPE cells treated with TGF-β2. (b) HMVECs were incubated to assess proliferation in hRPE-conditioned medium for 24 h. (c and d) HMVECs were seeded on BM matrix in hRPE-conditioned medium (mean±s.e.m.; n=6 per group). Tube formed by HMVECs were photographed after a 12-h incubation period (4 × objective). (b–d) HMVECs cultured in hRPE-conditioned medium transfected with tenascin-C siRNA after TGF-β2 stimulation showed a significant reduction in proliferation and tube formation compared with the control cells. Values are expressed as a percentage relative to the control. *P<0.05, **P<0.01, ANOVA/Tukey test.