Figure 1
Expression of erythropoietin (EPO) and hypoxia-inducible factors (HIFs) in kidneys with unilateral ureteral obstruction (UUO) and iron treatment. (a) Changes in EPO mRNA expression from kidney of sham-operated or UUO mice at day 7. Quantitative real-time reverse transcriptase-polymerase chain reaction (qRT-PCR) analysis of EPO mRNA expression. The values are expressed as means±s.d. **P<0.01 vs sham operation; n=12 in each group. (b) qRT-PCR analysis of HIF-2α and HIF-1α mRNA expression. The values are expressed as means±s.d. **P<0.05 vs sham operation; n=12 in each group. (c) Left panels; representative figures of HIF-2α and HIF-1α from kidney at 7 days after sham operation or UUO. Right panel: semi-quantitative analysis of densitometry for HIF-2α and HIF-1α protein expression. The values are expressed as means±s.d. *P<0.05, **P<0.01 vs sham operation; n=3 in each group. (d) Changes in EPO mRNA expression from kidney of vehicle- or SFO-treated UUO mice. qRT-PCR analysis of EPO mRNA expression. The values are expressed as means±s.d. **P<0.01 vs vehicle; n=11–13. (e) qRT-PCR analysis of HIF-2α and HIF-1α mRNA expression. The values are expressed as means±s.d. *P<0.05, **P<0.01 vs vehicle treatment; n=11–13 in each group. (f) Left panels; representative figures of HIF-2α and HIF-1α from kidney with vehicle or iron treatment. Right panel: semi-quantitative analysis of densitometry for HIF-2α and HIF-1α protein expression. The values are expressed as means±s.d. **P<0.01 vs vehicle treatment; n=12 in each group.
