Figure 2
(a) Representative hematoxylin and eosin staining of the kidney section. (b) Western blot analysis showing the effects of iron on PDGF-Rβ and α-SMA expression in kidney. Upper panels; representative figures of PDGF-Rβ from kidney with vehicle or SFO treatment. Lower panel: semi-quantitative analysis of densitometry for PDGF-Rβ expression. The values are expressed as means±s.d. *P<0.05 vs vehicle treatment; n=8 in each group. (c) Upper panels; representative figures of α-SMA from the kidney with vehicle or SFO treatment. Lower panel: semi-quantitative analysis of densitometry for α-SMA expression. The values are expressed as means±s.d. **P<0.01 vs vehicle treatment; n=4 in each group. (d) Iron-induced transition from fibroblast to myofibroblast in kidney. Representative immunohistochemistry of DAPI (blue), PDGF-Rβ (green), and α-SMA (red) staining. (e) SFO-increased labile ferrous iron in the kidney. Left panels; representative figures of RhoNox-1 staining in the kidney with or without SFO treatment. Right panel: semi-quantitative analysis of fluorescence intensity. The values are expressed as means±s.d. *P<0.05 vs vehicle treatment; n=4 in each group. (f) Iron-induced oxidative stress production in the kidney. Left panels; representative figures of dihydroethidium (DHE) staining in kidney with or without SFO treatment. Right panel: semi-quantitative analysis of fluorescence intensity. The values are expressed as means±s.d. **P<0.01 vs vehicle treatment; n=3 in each group. (g) Fluorescence image of lectin (green), E-cadherin (blue), RhoNox-1 (red), and merged image of the same field of the kidney section. (h) Fluorescence and light microscope images of DHE staining, and merged image of the same field of the kidney section. G, glomeruli.
