Figure 3
The effect of iron on EPO and HIFs expression in HepG2 cells. (a) HepG2 cell viability was significantly decreased only by 1000 μg/ml SFO treatment. The values are expressed as means±s.d. **P<0.01 vs vehicle treatment; n=8 in each group. (b) Impact of SFO on EPO mRNA expression and dose-dependency in HepG2 cells. The values are expressed as means±s.d. **P<0.01 vs vehicle treatment; n=4 in each group. SFO action on HIFs expression in HepG2 cells. (c) Quantitative analysis of HIF-2α and HIF-1α mRNA expression. The values are expressed as means±s.d. *P<0.05 vs vehicle treatment; n=6. (d) Left panels; representative figures of HIF-2α, HIF-1α, and tubulin with vehicle or SFO treatment. Right panel: semi-quantitative analysis of densitometry for HIF-2α and HIF-1α protein expression. The values are expressed as means±s.d. **P<0.01 vs vehicle treatment; n=6 in each group. (e) Suppressive effect of SFO on cobalt chloride-induced EPO mRNA upregulation in HepG2 cells. The values are expressed as means±s.d. *P<0.05, **P<0.01 vs vehicle treatment; n=8 in each group. (f) SFO suppressive action on cobalt chloride-induced upregulation of HIF-2α and HIF-1α protein expression in HepG2 cells. Upper panels; representative figures of HIF-2α, HIF-1α and tubulin. Lower panel: semi-quantitative analysis of densitometry for HIF-2α and HIF-1α expression. Values are expressed as means±s.d. *P<0.05, **P<0.01 vs vehicle treatment; n=9 in each group. (g) Inhibitive effect of SFO on DMOG-induced EPO mRNA upregulation in HepG2 cells. The values are expressed as means±s.d. *P<0.05 vs vehicle treatment; n=4 in each group. (h) SFO inhibitive effect on DMOG-induced upregulation of HIF-2α and HIF-1α expression in HepG2 cells. Upper panels; representative figures of HIF-2α, HIF-1α and tubulin. Lower panel: semi-quantitative analysis of densitometry for HIF-2α and HIF-1α expression. The values are expressed as means±s.d. *P<0.05, **P<0.01 vs vehicle treatment; n=8 in each group.
