Table 1 Sequence of the primers for real-time PCR

From: Iron suppresses erythropoietin expression via oxidative stress-dependent hypoxia-inducible factor-2 alpha inactivation

Target gene

Forward (5′-3′)

Reverse (5′-3′)

Mouse EPO

ATGTCGCCTCCAGATACCAC

CCTCTCCCGTGTACAGCTTC

Mouse HIF-2α

CTAAGTGGCCTGTGGGTGAT

CGAAGTCCTTTGCAGACCTC

Mouse HIF-1α

TCAAGTCAGCAACGTGGAAG

TATCGAGGCTGTGTCGACTG

Mouse VEGF

CAGGCTGCTGTAACGATGAA

GCATTCACATCTGCTGTGCT

Mouse PHD3

CAGGTTATGTTCGCCATGTG

TGGCGTCCCAATTCTTATTC

Mouse PGK1

GTCGTGATGAGGGTGGACTT

TTTGATGCTTGGAACAGCAG

Human EPO

TCCGAACAATCACTGCTGA

CCTCCCCTGTGTSCSGCTTC

Human HIF-2α

TGCTGGATTGGTTCACACAT

GGGCCAGGTGAAAGTCTACA

Human HIF-1α

TGCTCATCAGTTGCCACTTC

TCCTCACACGCAAATAGCTG

Human VEGF

AAGGAGGAGGGCAGAATCAT

CACACAGGATGGCTTGAAGA

Human PHD3

AGATCGTAGGAACCCACACG

TTCTGCCCTTTCTTCAGCAT

Human PGK1

GAAGTGGAGAAAGCCTGTGC

GCATCTTTTCCCTTCCCTTC

36B4

GCTCCAAGCAGATGCAGCA

CCGGATGTGAGGCAGCAG

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