Figure 5 | Laboratory Investigation

Figure 5

From: Mixed cell therapy of bone marrow-derived mesenchymal stem cells and articular cartilage chondrocytes ameliorates osteoarthritis development

Figure 5

Co-culture of hBMSCs and hACCs enhances cartilage phenotype in an ex vivo cartilage defect model. Following 3 weeks of growth in chondrogenic induction medium, cell pellets (ACCs, BMSCs, and ACCs+BMSCs) were placed in cartilage defect explants and sealed with matrigel before being implanted subcutaneously in SCID mice for further 3 weeks. (a) IHC staining was performed to assess expression of chondrogenic marker (ACAN and COL2), hypertrophic markers and degradative (COL10 and MMP-13) and angiogenesis factor (VEGF) and angiogenesis inhibitor (ChM-1) and de-differentiation marker (Col1). (b) Quantification of staining in the mixed ACCs+BMSCs group compared with the ACCs and BMSCs-only groups. Data are reported as means±s.d.; *P<0.05. Scale bar=50 μm. Results are representative of studies performed on three different patient samples with two technical replicates.

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